Coupon Evaluation Flow Cells

          The BST Coupon Evaluation Flow Cells are single or dual channel flow cells that use standard microscope coverslips as viewing windows. The flow chambers of the flow cells are made from either clear polycarbonate plastic or black anodized aluminum. Both material types are fully autoclavable and reuseable. Anodized aluminum provides a more rugged material to last through numerous autoclave cycles (all plastics, including polycarbonate, will eventually deteriorate with repeated autoclave cycles). The flow chambers are approximately 1.5” (39 mm) long by 0.5” (13 mm) wide by 0.014” (0.34 mm) deep. The flow chambers have recessed wells in the bottom of the flow channel to accommodate test coupon samples. Three different coupon configurations are available.

          These microscopy flow cells are constructed in a sandwich of aluminum compression plates, silicone rubber gaskets, and the polycarbonate or anodized aluminum flow channel. The flow cells have barbed fittings that accommodate size 16 (3 mm ID) tubing. Viewing windows are commercially available microscope cover slips (60 x 24 mm; no. 2). The use of cover slips as viewing windows combines high quality optical glass, thin dimensions (increases working focal depth into the chamber) and low cost. These viewing windows can be removed and replaced easily between experiments, providing access to the test coupon surfaces.

          The Coupon Evaluation Flow Cells are provided with a standard, platform-stage microscope mount. Alternate mounting plates are available to mount the flow cells onto a microtiter plate holder that are common on many upright and inverted microscopes. The stage mount allows direct attachment to upright and inverted microscope stages. The Coupon Evaluation Flow Cell allows biofilm imaging using transmitted, epi-fluorescence, or confocal microscopy.

          These flow cells are designed for a maximum flow rate of 3.5 mL/minute.